Figure 7From: Involvement of lipid rafts in adhesion-induced activation of Met and EGFRMet and EGFR are excluded from the plasma membrane enriched by lipid rafts upon cell adhesion. A431 cells were replated on glass coverslips coated with 100 μg/ml poly-L-lysine for 30 min and stained for lipid rafts with Alexa 488-conjugated cholera toxin B subunit. The cells were stained for Met (a) or EGFR (b) with a monoclonal antibody which specifically recognizes the extracellular domain of the receptors and visualized by confocal microscopy. Arrowheads on the XZ- section images indicate the height at which XY -section images were taken. Scale Bar, 5 μm.Back to article page