Figure 2

Annexin V analysis of apoptotic cells. Untransfected NPC-TW01 cells (blank), transfection reagent only (mock), transfection reagent and miR-negative control (miR-negative), and transfection reagent and miR-1 (miR-1) separately. After transfection for 90 hours, the cells were stained by annexin V and propidium iodide and detected by flow cytometry. The percentage of cells stained in each quadrant is listed in the corner of each quadrant. The upper left quadrants represent oncosis cells, lower left quadrants represent normal cells, upper right quadrants represent necrotic cells (late apoptotic or oncotic cells) and lower right quadrants represent early apoptotic cells.