Table 2 Oligonucleotides used for pmirGLO luciferase reporter vector construction
From: MicroRNA-1 induces apoptosis by targeting prothymosin alpha in nasopharyngeal carcinoma cells
Name | Sequences(5' to 3') |
|---|---|
PTMA#1-wt-se | ACGAGCTCTTGTATTTTTTATTTACATTTTATCTAGAG |
PTMA#1-wt-as | CTCTAGATAAAATGTAAATAAAAAATACAAGAGCTCGT |
PTMA#1-mut-se | ACGAGCTCTTGTATTTTTTATTATGTAATTATCTAGAG |
PTMA#1-mut-as | CTCTAGATAATTACATAATAAAAAATACAAGAGCTCGT |
PTMA#2-wt-se | ACGAGCTCAAACAATCTTATTCCGAGCATTCCATCTAGAG |
PTMA#2-wt-as | CTCTAGATGGAATGCTCGGAATAAGATTGTTTGAGCTCGT |
PTMA#2-mut-se | ACGAGCTCAAACAATCTTATTCCGAGGTAAGGATCTAGAG |
PTMA#2-mut-as | CTCTAGATCCTTACCTCGGAATAAGATTGTTTGAGCTCGT |
pmirGLO-sequencing-primer | TGAAGAGCCTGATCAAATAC |