Figure 4

Involvement of MAP kinases in eotaxin-1-induced MMP-3 gene expression and protein secretion in human chondrosarcoma cells. SW1353 cell were pretreated for 1 h with inhibitors as follows: ERK inhibitor PD98059 at 1, 5 and 10 μM (A), p38 inhibitor SB203580 at 0.1, 1 and 3 μM (B), and JNK inhibitor SP600125 at 10 and 20 μM (C). The cells were then treated with eotaxin-1 (100 ng/ml) or IL-1β (1 ng/ml) for 6 h. MMP-3 mRNA expression was assayed by RT-PCR. (D) MMP-3 protein secretion of the cells treated with the inhibitors was also analyzed by Western blotting and quantified. Concentrations of the inhibitors used from low to high: 10 and 20 μM PD98059, 5 and 10 μM SB203580, 2.5 and 5 μM SP600125. These experiments were performed at least three times. The values in the plot were the mean of at least three assays.