Figure 5
From: Regulation of MMP-3 expression and secretion by the chemokine eotaxin-1 in human chondrocytes
Involvement of AC/PKA in eotaxin-1-induced MMP-3 gene expression in human chondrosarcoma cells. SW1353 cell were pretreated with various concentrations of cAMP inhibitor Rp-cAMP (A) or PKA inhibitor H-89 (B) for 1 h, and then treated with eotaxin-1(100 ng/ml) or IL-1β (1 ng/ml) for 6 h. MMP-3 mRNA expression was assayed by RT-PCR. These experiments were performed three times.