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Figure 2 | Journal of Biomedical Science

Figure 2

From: Immortalized mesenchymal stem cells: an alternative to primary mesenchymal stem cells in neuronal differentiation and neuroregeneration associated studies

Figure 2

Construction, morphology and characteristics of rat immortalized mesenchymal stem cells (IMSCs). A: Schematic drawings of the integrating component of retroviral vector SSR#69 for reversible immortalization. SSR#69 contains the hygromycin B resistance gene (Hyg R) as a positive selectable marker and the herpes simplex virus thymidine kinase gene (HSV-TK) as a negative selectable marker. The SV40T, Hyg R and HSV-TK genes are flanked by loxP sites. B: Morphology of rat IMSCs at 40 passage (Scale Bar = 100 μm). C: Cultured IMSCs were stained with haematoxylin-eosin (HE) (Scale Bar = 100 μm). D: Western bloting was performed to determine the protein level of SV40T antigen in three different kinds of MSCs. SV40T antigen expressed highly in IMSCs, however, it almost could not be detected in IMSCs after Cre-recombination. MSCs did not express SV40T antigen.

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Journal of Biomedical Science

ISSN: 1423-0127