Effects of TIG1A and TIG1B expression on PGE2-stimulated HCT116 cell proliferation. (A) Western blot analysis of TIG1A, TIG1B, GRK5 and β-catenin expression in MFP (5 nM)-induced TIG1 and control (Ctrl) stable clones. (B) Effect of MFP on PGE2-stimulated growth of control stable cells. (C) Effects of TIG1A and TIG1B expression on PGE2-induced HCT116 cell growth. Representative results of three independent experiments are shown. *, P < 0.05; **, P < 0.01; ***, P < 0.001.