Figure 3
From: Phosphatidylcholine induces apoptosis of 3T3-L1 adipocytes
Effects of PPC, SD, and Lipobean® on apoptotic pathways. (A) After serum starvation, 3T3-L1 pre-adipocytes were treated with PPC, SD, and Lipobean® at 0.1 mg/ml for 4 h. Whole cell lysates were then subjected to Western blot analysis with antibodies against phospho-specific p38 MAPK and phospho-specific JNK. (B) After serum starvation, 3T3-L1 pre-adipocytes were treated with PPC, SD, and Lipobean® at 1 mg/ml for 24 h. Whole cell lysates were then subjected to Western blot analysis with antibodies against Bax, Bcl-2, caspase-9, caspase-8, cleaved caspase-3, and PARP. Equal protein loadings were confirmed using anti-actin antibody.