Figure 4

Effects of PPC, SD, and Lipobean^® on differentiated 3T3-L1 cells. 3T3-L1 pre-adipocytes were differentiated for 6 days with MDI media. (A) Differentiated 3T3-L1 cells were treated with PPC, SD, and Lipobean^® at 0-1 mg/ml. After 24 h, cell viability was measured by the MTT assay. The data represent the mean ± S.D. of triplicate assays expressed as percentages of the control. * P < 0.01 compared to the untreated control (B) Differentiated 3T3-L1 cells were treated with PPC, SD, and Lipobean^® at 0.1 mg/ml for 24 h. Cell cycle analysis of cells was performed by a flow cytometer. (C) Differentiated 3T3-L1 cells were treated with PPC, SD, and Lipobean^® at 1 mg/ml for 24 h. Whole cell lysates were then subjected to Western blot anaysis with antibodies against Bax, Bcl-2, caspase-8, and PARP. Equal protein loadings were confirmed using anti-actin antibody.