Figure 1
From: A novel role of sesamol in inhibiting NF-κB-mediated signaling in platelet activation
Effects of sesamol on IKKβ phosphorylation and IκBα degradation in collagen-activated platelets. Washed platelets (1.2 × 109/ml) were preincubated with or without sesamol (2.5~25 μM) or a solvent control (0.5% DMSO), followed by the addition of collagen (1 μg/ml) to trigger (A) IKKβ phosphorylation for 3 min or (B) IκBα degradation for the indicated times (3~30 min), and (C) IκBα degradation for 10 min. Cells were collected, and subcellular extracts were analyzed as described in "Methods". For the lactate dehydrogenase (LDH) experiment (D), washed platelets (3.6 × 108/ml) were preincubated with Tyrode's solution, solvent control (0.5% DMSO), and various concentrations of sesamol (5~100 μM) for 20 min at 37°C, a 10-μl aliquot of supernatant was deposited on a Fuji Dri-Chem slide LDH-PIII as described in "Methods". Data are presented as the means ± S.E.M. (n = 4). *p < 0.05 and ***p < 0.001, compared to the control group (resting); #p < 0.05, ##p < 0.01, and ###p < 0.001, compared to the collagen group.