Cyclic nucleotide-dependent inhibition of IKKβ phosphorylation, IκBα degradation, and p65 phosphorylation by sesamol in collagen-activated platelets. Washed platelets (1.2 × 109/ml) were preincubated with sesamol (2.5 and 25 μM) or a solvent control (0.5% DMSO) in the absence or presence of SQ22536 (100 μM), ODQ (20 μM), or H89 (5 μM), followed by the addition of collagen (1 μg/ml) to trigger (A) IKKβ phosphorylation for 3 min or (B and D) IκBα degradation and (C) p65 phosphorylation for 10 min. Cells were collected, and subcellular extracts were analyzed by immunoblotting. Data are presented as the means ± S.E.M. (n = 4). *p < 0.05 and ***p < 0.001, compared to the control group (resting); #p < 0.05, ##p < 0.01, and ###p < 0.001, compared to the collagen group.