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Figure 3 | Journal of Biomedical Science

Figure 3

From: Mislocalization of the exitatory amino-acid transporters (EAATs) in human astrocytoma and non-astrocytoma cancer cells: effect of the cell confluence

Figure 3

Cell fractionation of primary and malignant astrocytes. Sub-confluent primary astrocytes and STTG-1 cells were subjected to cell fractionation and western-blot analysis as explained in Materials and Methods. Nitrocellulose membranes were probed with antibodies directed against EAAT1/GLAST or EAAT2/GLT-1 or against antigens specific of each compartment (i.e. Hsp90, EGF-R and Histone 3 for the cytosolic, membrane and nuclear fractions, respectively). Cy = cytosolic fraction, Mb = membrane fraction, Nu = nuclear fraction.

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