Figure 5

Quantification of the nuclei-associated EAATs signal in sub-confluent and confluent human cancer cells. Sub-confluent (white bars) and confluent (dark bars) human cancer cells were labelled for EAAT1/GLAST or EAAT2/GLT-1. The percentage of the GLAST (A) or GLT-1 (B) signal that co-localised with DAPI was quantified using ImageJ as explained in Materials and Methods. Results obtained by quantification of at least 20 different cells were expressed as means ± standard deviation (SD). Statistical difference between sub-confluent and confluent cells was evaluated using t-test with * p < 0.05 and ** p < 0.01.