Journal of Biomedical Science

Table 3 Net transport of L-glutamate by human cancer cells and rat astrocytes.

From: Mislocalization of the exitatory amino-acid transporters (EAATs) in human astrocytoma and non-astrocytoma cancer cells: effect of the cell confluence

	*Sub-confluent cells*			*Confluent cells*
	*control*	*+ PDC*	*+ Sulfa*	*control*	*+ PDC*	*+ Sulfa*
AGS	+ 32 (± 15)	+ 39 (± 21)	*- 9.1 (± 13)*	- 74 (± 6.1)	*+ 41 (± 18)*	- 81.2 (± 8.4)
Caco-2	+ 36 (± 26)	+ 44 (± 18)	*- 4.5 (± 9.2)*	- 69.9 (± 9.6)	*+ 42 (± 12)*	-72.6 (± 9.6)
HeLa	+ 27 (± 12)	+ 39 (± 9.8)	*- 7.8 (± 11)*	- 63.2 (± 5.8)	*+ 47 (± 18)*	- 69.1 (± 5.2)
HOG	+ 37 (± 9.6)	+ 41 (± 12)	*- 2.3 (± 3.4)*	- 68.4 (± 9.4)	*+ 52 (± 17)*	-71.7 (± 6.8)
HT29	+ 48 (± 14)	+ 51 (± 7.5)	*- 5.4 (± 9.1)*	- 64.1 (± 14)	*+ 49 (± 9.8)*	- 69.8 (± 5.9)
SHSY5Y	+ 15 (± 9.9)	*+ 34 (± 6.4)*	*- 13 (± 6.8)*	- 74 (± 1.8)	*+ 48 (± 13)*	- 79.2 (± 9.4)
STTG-1	+ 52 (± 24)	+ 62 (± 13)	*- 6.9 (± 3.4)*	+ 69 (± 12)	+ 74 (± 9.5)	*- 9.1 (± 7.9)*
Astrocytes	- 79 (± 2.1)	*+ 14 (± 10.9)*	- 82 (± 3.4)	- 91.7 (± 2.7)	*+ 21 (± 12)*	- 95.6 (± 9.1)

Net transport of L-glutamate was measured as explained in Materials and Methods. Results were expressed as variations of the extracellular concentration of L-glutamate after 6 h of incubation at 37°C. Initial concentration of L-glutamate in cell medium was 100 μM. Positive and negative variation values corresponded to net secretion and absorption of L-glutamate, respectively. Inhibitors used were PDC at 100 μM and sulfasalazine at 250 μM. All results were obtained from three independent experiments and were expressed as means of the variation (in μM) ± standard deviation (S.D). Values in bold are statistically different from the control values with p at least < 0.05.

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ISSN: 1423-0127

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