Figure 8

Tubby domain of Ktub is critical for mediating rhodopsin endocytosis. Confocal images show Rh1 endocytosis in photoreceptor cells. Flies received light stimulation for three hours and then processed for immunostaining with anti-Rh1 antibody and rhodamine-phalloidin. In wild type (A), some RLVs appeared in the cytoplasm of photoreceptor cells. In rh1 > ktub flies, massive RLVs appeared in the cytoplasm of photoreceptor cells (B). rh1 > C-ktub flies, many RLVs appeared in the cytoplasm of photoreceptor cells (C). In rh1 > N-ktub flies, only a few RLVs appeared in the cytoplasm of photoreceptor cells (D). The scale bar is 10 μm.