Figure 6

(a) Left panel: Western blot analysis of the levels of phosphoserine 897 on the GluN1 subunit (pGluN1-serine 897, top) and GluN1 subunit (GluN1, bottom) in lateral horn regions of spinal cord at 10 min after intrathecal injection of saline (control) and cAMPS-Sp (0.5 and 5 nmol). The percentage changes in the ratio of pGluN1-serine 897 to GluN1 are shown in the right panel. Values denote mean + SEM from three separate experiments. *Significant difference from control (100%). (b) Representative recordings of changes in blood pressure induced by an intrathecal injection of NMDA (2 nmol) before (control) and at 10 min and 40 min after intravenous injection of ketamine (2 mg/kg, left panel; 4 mg/kg, right panel) following pretreatment with cAMPS-Sp (5 nmol); cAMPS-Sp was applied intrathecally 10 min before the administration of ketamine. (c) Bar graphs show percentage changes in NMDA-induced increases in MAP 10 min and 40 min after intravenous injection of ketamine (2 mg/kg, left part; 4 mg/kg, right part) following intrathecal pretreatment with various doses of cAMPS-Sp. *Statistically significant difference from ketamine alone group (without pretreatment with cAMPS-Sp) analyzed using two-way ANOVA followed by Bonferroni post-test.