Figure 1

Schematic diagram and genotyping of AluYa5 insertion. A) Schematic of mono-allelic Alu Ya5 insertion in Chromosome 11. The size of Alu-present allele is 476 bp and Alu-absent allele is 150 bp. The red line is Alu Ya5 inserted in human genome. Black circles represent CpG sites. The location of PCR primers are represented in blue arrows. B) The genotyping of Alu insertion. The genomic DNA from six cell lines, Raji, Jurkat, HT15, H1299, MCF, and K562, are amplified with PCR primer marked above. Single 150 bp bands are generated from bi-allelic Alu-absent cell lines, 150 bp and 476 bp are from mono-allelic inserted cell lines, and single 476 bp are produced from bi-allelic inserted cell line.