Figure 2

Alu insertion dependent restriction enzyme digestion. A) Predicted size and cutting site after restriction enzyme digestion. B) Agarose gel electrophoresis and C) density of gel bands. Bisulfite-PCR products from mono-allelic Alu inserted cell lines, HT15 and H1299, are digested with HpyCH4III restriction enzyme. 'Uncut' represent a pre-digestion of PCR products which show the intact Alu-present (orange line and arrow heads) and Alu-absent (dark blue line and arrow heads). 'HpyCH4III' represent a post-digestion of PCR products which generate 72 bp bands (light blue lines and arrow heads) and either 404 bp (green lines and arrow heads) or 78 bp (red lines and arrow heads), depends on the DNA methylation status in the CpG site. The density of the gel band from Alu-present allele was measured.