Figure 2

Pro-caspase-3 physically associated with cytoplasmic p21 induction by p65 in PANC1 cells. (A) Cells were transiently transfected with or without over-expression of p65 and p65-targeted shRNA followed by DOX treatment (2 μg/ml) for 24 h. Whole cell extract was subjected to immunoprecipitation with anti-p21 antibody and then immunoblotted with anti-pro-caspase-3 or -active-caspase-3 antibody. (B) Cells were transfected with vector expressing wild-type (1-585), N-terminal (1-372) and C-terminal (373-585) region of p21 followed by DOX treatment (2 μg/ml) for 24 h. Immunoprecipitation with an antibody against myc peptide. The pro-caspase-3 and p21 amounts were assessed by Western blotting.