Change of P65 status was not related cell cycle pattern in PANC1 cells. Cells were transiently transfected with or without over-expression of p65 and p65-targeted shRNA followed by DOX treatment (2 μg/ml) for 24 h. (A) The level of CDK2, CDK4, and cyclinD1 or cyclinE protein were detected by Western blotting. GAPDH was used as controls. (B) Cell cycle pattern. Error bar indicates the standard error of the mean of three independent experiments.