Lutein affects cellular components. (A) Cells were incubated with vehicle or lutein (10 μM) for 16 h and cell viability was measured by MTT assay as described in Methods. (B) Cells were preincubated with vehicle or lutein (10 μM) for 1 or 6 h and followed by an extensive wash to remove extracellular unbound lutein. Cells were then stimulated with PBS or PDGF for 10 min. PDGFR and PLCγ phosphorylation was examined by Western blotting and quantitation was performed by densitometry (n = 3).