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Figure 2 | Journal of Biomedical Science

Figure 2

From: Lipid raft/caveolae signaling is required for Cryptococcus neoformans invasion into human brain microvascular endothelial cells

Figure 2

Effect of Cav1 siRNA on HBMEC. (A) A Western blot shows the Cav1 proteins from HBMEC with the following treatments: lane 1: mock; lane 2: random oligonucleotides; and lane 3: Cav1 siRNA. (B) An immunofluorescence microscopic image shows the reduction of Cav1 in the siRNA-treated HBMEC. Most cells show only DAPI stain, and one HBMEC still has some Cav1 signals (red). (C) The membrane raft marker ganglioside GM1 of HBMEC was stained with CTxB-FITC (green) and a C. neoformans cell (strain B-4500FO2) was stained with anti-GXM monoclonal antibody and rhodamine conjugate (red) (lower panel). The upper panels (Mock) are the controls without incubation with C. neoformans cells (strain B-4500FO2) (+Cn cells). The right column is HBMEC with Cav1 siRNA treatment (Cav1-KD) vs. the control (left column). (D) The Metamorph program associated with our fluorescence microscope was used to scan images with 5~10 random fields, and then used the statistical package GradPad Prime 5 to quantitate the readings. Analysis of variance shows significant increases in the GM1 signals in the presence of C. neoformans cells (P = 0.0029), but no different in the siRNA treated samples.

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