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Figure 7 | Journal of Biomedical Science

Figure 7

From: Lipid raft/caveolae signaling is required for Cryptococcus neoformans invasion into human brain microvascular endothelial cells

Figure 7

Induction of Cav1 phosphorylation mediated by the HA-CD44 pathway. (A) HBMEC were treated with 106 C. neoformans strains B-4500FO2 (CPS1+) or C559 (cps1 Δ), or treated with 1 and 2 μg hyaluronic acid (HA) for 10 minutes (A). The cell lysates were prepared and immunoblotted for the detection of the phosphorylated form of Cav1 (top row). β-actin was used as the loading control (second row). The quantitative and graphic analyses (lower panel) were described in the legend of Figure 6. (n = 4) (B) HBMEC cells were pre-treated with filipin (0 to 5 ng) for 10 minutes prior to the treatment of 106 C. neoformans strain B-4500FO2 cells. The signals of Pi-Cav1 were then examined as described above.

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