Figure 4
From: siRNA against presenilin 1 (PS1) down regulates amyloid β42 production in IMR-32 cells
Immunofluorescence analysis of Aβ42 in methanol acetone fixed IMR-32 cells after transfected with PS1 siRNA and control siRNA. Plane 1: Non siRNA transfected IMR-32 Cells; Plane 2: Control siRNA; Plane 3: 50 pmoles PS1 siRNA; Plane 4: 75 pmoles PS1 siRNA; Plane 5: 100 pmols PS1 siRNA. PS1 siRNA and control siRNA transfected IMR-32 cells were probed with Aβ42 antibody. PS1 siRNA and control siRNA transfected IMR-32 cells were probed with anti Aβ42 antibody. The signal was revealed using FITC-conjugated secondary antibody and visualized on a fluorescence microscope (o.m. 10X).