Table 2 Solvent accessibilities and Secondary structure analysis in the native and mutant proteins
Mutation Position | Solvent accessibility in the native and mutant proteins by GETAREA | Secondary structure analysis by DSSP | |
|---|---|---|---|
| Â | Changed from exposed to buried | Changed from buried to exposed | Â |
W274C | Thr (2), Tyr(5) Leu(7), Val(63), His(76), Ala(81), Pro(93), Ser(116), Ala(119), Glu(129), Lys(146), Tyr(155), Lys(185), Leu(187), Ala(194), Lys(213), Leu (217), Ile(310), Leu(319), Leu(327), Gln(335), Glu(340), Lys(344), Pro(349), Lys(399), Thr(400), Leu(419), Tyr(426), Lys(431), Lys(441), Ala(449), Thr(454), Lys(485), Arg(509), Gly(539), Thr(549), Ser(553), Glu(559), Glu(576), Asp(579), Gln(585), Lys(589), Val(592), Phe(598), Arg(602), Leu(614), Asn(631), Tyr(639), Ser(647), Ala(654), His(679), Leu(689), Val(697), Asn(713), Arg(719), Ser(728), Lys(732) | Trp(14), Leu(69), Gln(96), Val(99), Ser(138), Ser(176), Ser(179), Ile(405), Asn(299), Cys(329), Val(392), Arg(424), Tyr(473), Arg(503), Arg(546), Val(556), Pro(569), Gly(565), Asn(609), Gly(619), Ile(636), Trp(707) | T → H: Pro(86), Gly(89), Leu(203), Thr(208), Ser(428), Lys(518), Gly(638), His(1735), Thr(1763), Lys(1932), Asn(1934), Met(1945), Glu(2200) H → T:Lys(161), Lys(185), Leu(187), Leu(562), Asn(601), Glu(608), Arg(612), Ser(630), Asp(1865), Gly(2022) T → S:Trp(87), Leu(517), Asn(637), Asn(1772) |
W2065R | Asp(1260), Gln(1336), Leu(1481), Ala(1610), Ile(1698), Tyr(1699), Arg(1708), Thr(1714), Glu(1723), Arg(1740), Gly(1769), Leu(1775), Ile(1782), Arg(1800), His(1867), Leu(1882), Trp(1908), Ala(1939), Asn(1941), Met(1945), Arg(1960), Ser(1968), Asn(1971), Phe(1982), Met(2007), Arg(2016), Ser(2082), Arg(2169) | Asn(1460), Gln(1705), Gly(1779), Leu(1808), Val(1876), Glu(1884), Glu(1904), Met(1842), Ile(1901), Tyr(1909), Thr(2015), | T → H:), His(1735), Thr(1763), Lys(1932), Asn(1934), Met(1945), Glu(2200) H → T: Asp(1865), His(1867), Asp(2206) T → S: Asn(1772) |
W2248C | Asp(1260), Gln(1336), Asn(1460), Leu(1481), Ala(1610), Ile(1698), Tyr(1699), Arg(1708), Thr(1714), Glu(1723), Arg(1740), Gly(1769), Leu(1775), Ile(1782), Arg(1800), His(1867), Val(1876), Leu(1882), Trp(1908), Ala(1939), Asn(1941), Met(1945), Arg(1960), Ser(1968), Asn(1971), Phe(1982), Met(2007), Arg(2016), Gly(2028), Ala (2070)Ser(2082), Arg(2169) | Asn(1460), Gln(1705), Gly(1779), Leu(1808), Val(1876), Glu(1884), Glu(1904), Met(1842), Ile(1901), Tyr(1909), Thr(2015), Gly(2022), Ala(2070) | T → H:), His(1735), Thr(1763), Lys(1932), Asn(1934), Met(1945), Glu(2200) H → T: Asp(1865), Gly(2022) |