Figure 6
From: Anti-inflammatory effects of spermidine in lipopolysaccharide-stimulated BV2 microglial cells
Effects of spermidine on Akt and MAPKs activation induced by LPS in BV2 microglia. Cells were treated with different concentrations of spermidine 1 h before LPS treatment for the indicated times. Total proteins (50 μg) were separated on 10% SDS-polyacrylamide gels, followed by Western blotting using the indicated antibodies. Results are representative of those obtained from three independent experiments.