Figure 6

Effect of L-carnitine on expression levels of PPAR-α and its target genes in H ₂ O ₂ -treated HL7702 cells. HL7702 cells were pretreated with L-carnitine for 12 h and followed by the treatment of H₂O₂ (300 μM) for 12 h. A: Cells were collected, total RNA and protein were prepared to determine the mRNA and protein levels of PPAR-α in HL7702 cells using RT-PCR and western blot analysis respectively. B: Total RNA was extracted and expressions of CPT1 and ACOX were quantified using RT-PCR. The data was normalized to GAPDH expression. Each value represents the mean of three replicates. ^#P < 0.01 vs. control; *P < 0.05, **P < 0.01 vs. H₂O₂ alone.