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Table 2 Monoclonal antibody yield from protein A purification of mouse ascites

From: Monoclonal antibodies targeting the synthetic peptide corresponding to the polybasic cleavage site on H5N1 influenza hemagglutinin

Clone Content1 (μg/μL) Volume(mL) Yield2 (mg) Deglycerol3 (μg/μL)
A 14.5 2 18.0 10.9
B 1.1 0.2 0.2 0.1
C 18.0 1.7 30.0 17.4
D 3.3 0.2 0.7 0.5
3C4 56.0 1.1 59.0 31.8
4H2 9.9 0.4 3.8 5.7
6B8 50.0 0.6 27.9 28.3
  1. 1Monoclonal antibody contents in selected best fractions after protein A purification and Centricon enrichment before the addition of 50% glycerol. The immunoglobulin content was determined by Bradford method with BSA as the standard.
  2. 2The yield was obtained by multiplying content (μg/μL) with the volume (mL).
  3. 3The mAb were stored at -;80°C freezer in the presence of 50% glycerol. The glycerol can interfere with the virus infection assay, so it is removed by another round of protein A purification and the immunoglobulin content was determined again