Clone | Content1 (μg/μL) | Volume(mL) | Yield2 (mg) | Deglycerol3 (μg/μL) |
|---|
A | 14.5 | 2 | 18.0 | 10.9 |
B | 1.1 | 0.2 | 0.2 | 0.1 |
C | 18.0 | 1.7 | 30.0 | 17.4 |
D | 3.3 | 0.2 | 0.7 | 0.5 |
3C4 | 56.0 | 1.1 | 59.0 | 31.8 |
4H2 | 9.9 | 0.4 | 3.8 | 5.7 |
6B8 | 50.0 | 0.6 | 27.9 | 28.3 |
- 1Monoclonal antibody contents in selected best fractions after protein A purification and Centricon enrichment before the addition of 50% glycerol. The immunoglobulin content was determined by Bradford method with BSA as the standard.
- 2The yield was obtained by multiplying content (μg/μL) with the volume (mL).
- 3The mAb were stored at -;80°C freezer in the presence of 50% glycerol. The glycerol can interfere with the virus infection assay, so it is removed by another round of protein A purification and the immunoglobulin content was determined again
