1Monoclonal antibody contents in selected best fractions after protein A purification and Centricon enrichment before the addition of 50% glycerol. The immunoglobulin content was determined by Bradford method with BSA as the standard.
2The yield was obtained by multiplying content (μg/μL) with the volume (mL).
3The mAb were stored at -;80°C freezer in the presence of 50% glycerol. The glycerol can interfere with the virus infection assay, so it is removed by another round of protein A purification and the immunoglobulin content was determined again