Efficient knockdown of OLFM4 by RNA interference in gastric cancer SGC-7901 and MKN45 cells. A. Expression of OLFM4 protein in various gastric cancer cell lines. Expression profile of OLFM4 protein was analyzed using western blotting with β-actin as an internal control. GES-1 cells were served as normal control cells. B. OLFM4 mRNA expression in OLFM4 knockdown cells. Relative expression of OLFM4 was detected by qRT-PCR. β-actin was used as an internal control. Fold changes in OLFM4 mRNA expression were determined using the 2-ΔΔCt method. C. OLFM4 protein expression in OLFM4 knockdown cells. OLFM4 protein expression was detected by western blotting. β-actin protein was used as an internal control. Representatives of three experiments are shown. **P < 0.01 vs. HK control cells group (n = 3).