knockdown of OLFM4 inhibits the growth of SGC-7901 and MKN45 cells in vitro and in vivo. A. Cell growth curve. Cell proliferation in vitro was assessed by cell growth curve, as determined by counting the cell number (WST-1 assay) in the SGC-7901 cells (left panel) and MKN45 cells (right panel). The OD value (450 nm) was counted on the indicated days and presented as the mean cell numbers (n = 3). B. Anchorage-independent growth in soft agar. Representative images of three experiments were shown (upper panel). Data represent the mean number of colonies counted at 200 × magnification for 5 random fields (lower panel). C. The mean tumor volume after subcutaneous injection of nude mice with HK control or OLFM4 knockdown cells was measured at the indicated time points. D. Representative tumors images at 35 days after subcutaneous injection of indicated cells. E. Mean tumor weight (left panel) and inhibitory rate (right panel) in tumor xenografts. Data represent the mean tumor weight of xenografts (mean ± SD, n = 10). **P < 0.01 vs. HK control group.