qRT-PCR and IHC detection of OLFM4 in tumor xenografts. A. qRT-PCR for OLFM4 mRNA in tumor xenografts. Fold changes in OLFM4 mRNA were determined using the 2-ΔΔCt method. β-actin gene was used as an internal control. B. H&E staining (upper panel) and IHC for OLFM4 protein (lower panel) in tumor xenografts. Representative images (200×) are shown. N, Necrosis. C. Quantification analysis of OLFM4 expression. The average value was measured from five randomly different fields under the microscope (400×) using the Image-Pro PLUS V6.0. Data were expressed as mean ± SD. **P < 0.01 vs. HK control group.