Assessment of cell cycle distribution, apoptotic cells and caspase-3/9 activation in OLFM4 knock down gastric cancer cells. A. Cell cycle analysis. Changes in cell cycle distribution of SGC-7901-HK, SGC-7901-siOLFM4 and MKN45-HK, MKN45-siOLFM4 cells were analyzed by FCM. Data represent the mean percentage of cell-cycle phase distribution (n = 3). B. Cell apoptosis analysis. Apoptosis was estimated with AnnexinV-PE/7-AAD staining by FCM. Data represent the mean apoptotic cell percentage (n = 3). C. Caspase-3 and -9 activations. Caspase-3, 9 activations between indicated cells are shown. **P < 0.01 vs. HK control group.