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Figure 5 | Journal of Biomedical Science

Figure 5

From: Down-regulation of PKCζ in renal cell carcinoma and its clinicopathological implications

Figure 5

Inhibition of PKCζ expression led to increased chemoresistance and cell proliferation in HK-2 cells. (A) siRNA-mediated suppression of PKCζ gene expression in HK-2 cells. PKCζ siRNA (100 nM) were transfected into parental HK-2 cells. Note, the level of targeted PKCζ gene was significantly reduced by > 50% using RT-PCR analysis. (B) Similarly, the level of PKCζ protein was significantly inhibited by 57% at 100 nm siRNA using Western blotting analysis. C: control group, lipofectamine only, and α-tubulin was used as an internal control. (C) Parental HK-2 cells were treated 100 nm PKCζ siRNA (HK-2-siRNA) or lipofectamine only (HK-2-C), respectively for 24 hours. Chemosensivity of HK-2-C (IC50 = 2.05 μM) and HK-2-siRNA (IC50 = 4.02 μM) cells to cisplatin were examined by MTT assay. The IC50 value was a near 2-fold increase in HK-2-siRNA compared to HK-2-C. Similar results, chemosensivity of HK-2-C (IC50 = 0.09 μM) and HK-2-siRNA (IC50 = 0.17 μM) cells to paclitaxel were examined by MTT assay. The IC50 value was a near 2-fold increase in HK-2-siRNA compared to HK-2-C. P* < 0.05, P** < 0.005 and P*** < 0.001. (D) Parental HK-2 cells were treated with 0.16 μM PKCζ pseudosubstrate. After 60 h, cell proliferation was assessed by MTT assay. Points, means of two experiments plated in replicates of six; Data are presented as the mean ± standard error of the means (SEM). P* < 0.05, compared with untreated cells.

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