Primers | Sequence of primers | PCR amplification conditions |
---|---|---|
StAR | Sense:5'-CTCAACAACCAAGGAAGGCTGG-3' | 94°C, 60 s; 56°C, 40 s; |
 | Atisense:5'-GCAGGTGGGGCCGTGTTCAGC-3' | 72°C, 40 s; 30 cycles |
3-beta-HSD | Sense:5'-ACTGGCAAATTCTCCATAGCC-3' | 94°C, 30 s; 60°C, 45 s; |
 | Atisense:5'-GCTGAACAGTCGACCCTCCTT-3' | 72°C, 60 s; 30 cycles |
CHOP | Sense:5'-TCTGCCTTTCGCCTTTGAG-3' | 94°C, 40 s; 55°C, 60 s; |
 | Atisense:5'-GCTTTGGGAGGTGCTTGTG-3' | 72°C, 40 s; 29 cycles |
Bip | Sense:5'-CATCAATGAGCCAACAGC-3' | 94°C, 45 s; 60°C, 60 s; |
 | Atisense: 5'-AGGTAGAGCGGAACAGG-3' | 72°C, 45 s; 26 cycles |
PERK | Sense: 5'-GCCGATGGGATAGTGATG-3' | 94°C, 45 s; 62°C, 60 s; |
 | Atisense: 5'-GCAGCCTCTACAATGTCTTCT-3' | 72°C, 45 s; 28 cycles |
GAPDH | Sense:5'-GCTGGGGCTCACCTGAAGG-3' | 94°C, 45 s; 56°C, 60 s; |
 | Atisense:5'-GGATGACCTTGCCCACAGCC-3' | 72°C, 60 s; 30 cycles |