|
StAR
|
Sense:5'-CTCAACAACCAAGGAAGGCTGG-3'
|
94°C, 60 s; 56°C, 40 s;
|
| |
Atisense:5'-GCAGGTGGGGCCGTGTTCAGC-3'
|
72°C, 40 s; 30 cycles
|
|
3-beta-HSD
|
Sense:5'-ACTGGCAAATTCTCCATAGCC-3'
|
94°C, 30 s; 60°C, 45 s;
|
| |
Atisense:5'-GCTGAACAGTCGACCCTCCTT-3'
|
72°C, 60 s; 30 cycles
|
|
CHOP
|
Sense:5'-TCTGCCTTTCGCCTTTGAG-3'
|
94°C, 40 s; 55°C, 60 s;
|
| |
Atisense:5'-GCTTTGGGAGGTGCTTGTG-3'
|
72°C, 40 s; 29 cycles
|
|
Bip
|
Sense:5'-CATCAATGAGCCAACAGC-3'
|
94°C, 45 s; 60°C, 60 s;
|
| |
Atisense: 5'-AGGTAGAGCGGAACAGG-3'
|
72°C, 45 s; 26 cycles
|
|
PERK
|
Sense: 5'-GCCGATGGGATAGTGATG-3'
|
94°C, 45 s; 62°C, 60 s;
|
| |
Atisense: 5'-GCAGCCTCTACAATGTCTTCT-3'
|
72°C, 45 s; 28 cycles
|
|
GAPDH
|
Sense:5'-GCTGGGGCTCACCTGAAGG-3'
|
94°C, 45 s; 56°C, 60 s;
|
| |
Atisense:5'-GGATGACCTTGCCCACAGCC-3'
|
72°C, 60 s; 30 cycles
|
