Figure 1

EGFR protein and gene status in clear cell renal cell carcinoma. Immunohistochemical expression of EGFR protein, shown at lower (×10) and higher magnification (×20), was designated as discontinuous or continuous membrane staining of different intensity: (a) weak and moderate (+/++) discontinuous and continuous membrane immunostaining, and (b) strong continuous immunostaining (+++);. (c) chromosome 7 copy number was analyzed in tumor cells using fluorescence in situ hybridization (FISH) with an α-satellite DNA probe for chromosome 7 (centromere 7, green signal; EGFR gene, red signal); tumor nuclei showed disomy of chromosome 7 without EGFR gene amplification (d) tumor nuclei showed polysomy with a greater number of red and green signals than in normal cells; (c and d magnification × 100).