Figure 1

Concentrations of IL-12 and IFN-γ in right (R) and left (L) hemispheres of mice treated with rAAV2/GFP (n = 14) or rAAV2/IL-12 (n = 14). All mice were not implanted with tumor as indicated by (no). We measured both IFN-γ and IL-12 at the same time. IFN-γ (A) and IL-12 (B) levels, as expressed in picogram in 1 mg extracted protein, were measured by ELISA on the last day of week 0, 1, 2, 3, 4, 6, 8 (n = 2 each week) after injection of the virus vectors. All virus vectors were injected on the right hemisphere for this and the following experiments. For the following experiments in tumor implantation, 500 cells of RG2 (GBM cell line) were injected into the brain 1 mm anterior to the vector injection site on the last day of week 2 after the viral injection.