Reverse transcriptase and real-time PCR analysis of the expression of pancreatic β-cell development-related genes and insulin production-related genes. (A) Reverse transcriptase -PCR results for non-differentiated cells (MSCs, lane 1 and 2), differentiated cells (IPCs, lane 3 and 4). (B) Real-time PCR analysis of the expression of Pax4, Pax1 and Insulin. Results are expressed as the mean ± SEM for 4 experiments. *, P ≪ 0.05 compared to the non-differentiated cells.