Figure 6

Maintained polyubiquitination and preserved proteasomal activity in RVLM during experimental brain death. Illustrative gels or summary of phasic fold changes against sham-controls in polyubiquitinated proteins detected by Western blot analysis (A) or chemotrypsin-like (B), trypsin-like (C) and caspase-like (D) proteasome activity in RVLM of rats that received pretreatment by microinjection bilaterally into RVLM of lactacystin (Lacta; 1 nmol) or artificial cerebrospinal fluid (aCSF), followed by intravenous administration of LPS (15 mg kg^-1) or saline. Values are mean ± SEM, n = 5–6 animals per experimental group. *P < 0.05 versus aCSF + Saline group, and ⁺P < 0.05 versus aCSF + LPS group at corresponding phases in the Scheffé multiple-range test.