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Figure 4 | Journal of Biomedical Science

Figure 4

From: Identification and characterization of a novel gene, c1orf109, encoding a CK2 substrate that is involved in cancer cell proliferation

Figure 4

C1ORF109 is upregulated in multiple cancer cells and located in both the nucleus and cytoplasm. (A) C1ORF109 expression in 11 human breast cancer cell lines and one melanoma cell lines were detected by western blot analysis. The non-tumorigenic epithelial cell line MCF 10A and an immortalized human keratinocyte cell line HaCaT were used as control. (B) Immunofluorescence analysis showed the subcellular localization of C1ORF109 using an anti-Flag tag antibody. (C) Immunoelectron microscope with colloidal gold showed the subcellular localization of C1ORF109 using an anti-V5 epitope antibody. Arrows indicate the specific binding of gold particles. Nu, nucleus. C, cytoplasm. (D) The subcellular localization of endogenous C1ORF109 was detected in HeLa cells using anti-C1ORF109 antibody.

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Journal of Biomedical Science

ISSN: 1423-0127