Table 1 Sequences of oligonucleotides used in promoter cloning and site-directed mutagenesis
Oligonucleotide | Sequence (5′ to 3′) | Purpose |
|---|---|---|
PR | CGAGATCTCGTGCCTGGCTACTGAGTCGC | promoter cloning |
PF-1795 | CGACGCGTCGAGTTGTGGTCCAGGCTTGTTTCCC | promoter cloning |
PF-428 | CGACGCGTCGTTCCAGCCTCTCGGTTTCAGGG | promoter cloning |
PF-216 | CGACGCGTCGCTAACAGGACATGCCACCAC | promoter cloning |
PF-177 | CGACGCGTCGCCGCAGGCTGACAAATGAGAAG | promoter cloning |
PF-93 | CGACGCGTCGCCACATGTTGGACTACAGTAC | promoter cloning |
CAAT I mutR | TGGGACTGGATGTTGGGACCG | mutagenesis |
CAAT II mutR | TTAAACTGGGTGGCGGTGGTG | mutagenesis |
CAAT III mutR | GACACTGTGTATCACAACCAACTGGC | mutagenesis |
TATA mutR | CGAGATCTCTGCCTGGCTACTGAGTCGCGAAAATCTCTCGTAGTG | mutagenesis |
ChIP1F | AGAGCGGCTCTACAGTCAAC | ChIP |
ChIP1R | TATTGCAGAGCCGCCACAAGGC | ChIP |
ChIP2F | GAATGATAGAGGAGCAGG | ChIP |
ChIP2R | ATCCTCAGGCACCCAGCAGAC | ChIP |
ChIP3F | TTCCAGCCTCTCGGTTTCAGGG | ChIP |
ChIP3R | CTGCCTGGCTACTGAGTCGCGA | ChIP |
ChIP InpF | GGGTTCTCACGCTTTGGCTGTC | ChIP |
ChIP InpR | CCGCTCTTTTAAATCTGGGA | ChIP |
GC box | ACCCGGCTCCGCCCTGGCCGGCT | EMSA |