Figure 5

Effects of DATS on levels of Bcl-2 and IAP family proteins, and MMP values in U937 cells. (A) Cells were treated with the indicated concentration of DATS for 48 h. Cells were lysed and then equal amounts of cell lysates (30 μg) were separated on SDS-polyacrylamide gels and transferred to nitrocellulose membranes. Membranes were probed with the indicated antibodies and the proteins were visualized using an ECL detection system. Actin was used as an internal control. (B) Cells grown under the same conditions as (A) were stained with JC-1 and incubated at 37°C for 20 min. Mean JC-1 fluorescence intensity was detected using a flow cytometer. Data represent the means of two independent experiments.