Knockdown of the Other Autophagy-related Genes and Heat Shock Protein 22 Do Not Result in Any Rough Eye Phenotype in Drosophila . RNAi knockdown of different autophagy-related genes by GMR-Gal4 show normal eye morphology and regular ommatidia (like the control in Figure 1, A-A”. (A-A”) Atg1, (B-B”) Atg4, (C-C”) Atg5, (D-D”) Atg8a, (E-E”) Atg9, (F-F”) Atg12, (G-G”) Atg18, and (H-H”) Hsp22. Optical micrograph (A-H) and SEM (A’-H’:300X; A”-H”:1500X). Genotypes: GMR-Gal4 in trans to the alleles indicated.