Starvation-induced Autophagosome Formation is Inhibited by RNAi-mediated Depletion of Atg7 but not Hsp27. (A, E, I, M) GFP-NLS labeled fat body cells circled by dotted line indicate the presence of UAS-Atg7RNAior UAS-Hsp27RNAigenerated by the FLP/FRT method. The cells outside of the circled dotted line are used as the control cells without UAS-Atg7RNAior UAS-Hsp27RNAi. (B, F, J, N) The distribution patterns of mcherry-Atg8a are shown under either fully-fed or starvation conditions. (C, G, K, O) The fat body cells are stained with DAPI. (D, L) The picture D is merged from panels A, B, C and L is merged from panels I, J, K under nutrient-rich conditions. (H, P) Picture H is merged from panels E, F, G and P is merged from M, N, O under starvation conditions. The distribution of mcherry-Atg8a puncta is dramatically altered in starved fat body cells (F, N) compared to those under nutrient-rich conditions (B, J). GFP-labeled cells expressing Atg7-RNAi markedly suppress mCherry-Atg8a puncta formation (F), but not in that of Hsp27 knockdown (N).