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Figure 4 | Journal of Biomedical Science

Figure 4

From: Autophagy-related gene 7 is downstream of heat shock protein 27 in the regulation of eye morphology, polyglutamine toxicity, and lifespan in Drosophila

Figure 4

Atg7 is Downstream of Hsp27 in the Attenuation of the Mild Polyglutamine Toxicity by 41Q, but the Overexpression of Both Genes Cannot Rescue the Longer Polyglutamine Tract Toxicity by 63Q. (A) Expression of UAS-41Q by GMR-GAL4 results in mosaic eyes. (B, D) Both the overexpression of Hsp27 and Atg7 rescue the mosaic eye by 41Q. (C, E) Under 41Q background, both the knockdown of Hsp27 and Atg7 generate comparable worsened mosaic eyes, whereas knockdown of Atg7 leads to a rough eye surface. (F) Overexpression of Atg7 as well as knockdown of Hsp27 improves the mosaic eye by 41Q. (G, M) The expressions of UAS-63Q by GMR-Gal4 produce similar rough eye phenotype. (H, J) Overexpression of Hsp27 or Atg7 cannot rescue the rough eye induced by 63Q. (I, K) Knockdown of Hsp27 or Atg7 in conjunction with 63Q does not cause further deterioration of the eyes. (L) Overexpression of Atg7 together with knockdown of Hsp27 does not alter the rough eye phenotype by 63Q. Genotypes: (A-F) GMR-Gal4; UAS-41Q in trans to the alleles indicated. (G-L) GMR-Gal4/Cyo; UAS-63Q in trans to the alleles indicated.

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Journal of Biomedical Science

ISSN: 1423-0127