Figure 2

The E. coli BL21 (DE3). PET3a ZBD Expression Profile of Recombinant ZBD. E. coli BL21 (DE3). PET3a ZBD transformed cells were grown at 37 0 C to OD600 reading 0.2 ~ 0.4, 10ul of total cell extracts from 200 μl of cells solibilized in 1% SDS sample buffer as control for before IPTG induction were prepared as method described (lane 3). After 2 hour 0.8 mM IPTG induction, 10ul of total cell extracts from 100 ul of cells were prepared as after IPTG incubation (lane 4). 10ul of total cell extracts from 100 μl of 2 hours IPTG inducing PET 3a mock transformation BL21 (DE3) cells was prepared as negative control (lane 5). The pellet after French press and inclusion bodies 0.1% triton X-100 wash steps were prepared (lane 7). Lane 1: Molecular weight standard; Lane2 and 6: Blank.