Figure 7From: A smallest 6 kda metalloprotease, mini-matrilysin, in living world: a revolutionary conserved zinc-dependent proteolytic domain- helix-loop-helix catalytic zinc binding domain (ZBD)The polymerization of the 6 kDa ZBD of MMP-7 in pentamer and Octamer demonstrate the significant proteolytic activities towards to the CM-transferrin substrate in CM-transferrin zymographic assay. 300 μg of craboxylmethylated transferrin (CM-transferrin) was co-polymerized with SDS-PAGE as a substrate gel for analyzing the MMP-7 activities in situ.Back to article page