Figure 4

Nociceptive and locomotor measurements. (A) Nociceptive response in unaffected (n = 5) and affected (n = 8) mice from 4 weeks to 7 months. A reduction of the latency in response to mechanical stimuli was detected in affected mice. (B) Tail-flick and hot plate tests. The affected mice had a significantly shorter latency in the tail-flick test than unaffected mice. The latency in the hot plate test was longer in affected mice but was not significantly different from unaffected mice. (C) Locomotor activity in affected and unaffected mice. Locomotion in both the vertical (V) and horizontal (H) directions was lower in mice with autoinflammation than in unaffected mice. (D) Paw prints of affected and unaffected mice in the CatWalk analysis. The arrow indicates the gait direction. Notice that the right paw of the affected mice has significantly smaller paw prints than unaffected mice. (E) Rotarod test. No significant difference was found between the unaffected mice (n = 8) and affected mice (n = 10). (F) Homecage scanning, including drinking, feeding, hanging, rearing, grooming, and walking. A significant difference was only found in grooming between affected mice (n = 10) and unaffected mice (n = 10). Abbreviations: Unaff, unaffected mice; Aff, affected mice; V, vertical event; H, horizontal event.