Figure 6

Pharmacologic inhibition of phosphoinositide-3-kinase (PI3K) and Akt-PKB decrease Ca²⁺current in HL-1 cell mouse cardiomyocytes. A. Effect of LY294002 (10 μM) on I/V plot of Ca²⁺ current compared with control (mean ± SEM; n = 3). Holding potential = −50 mV. Inset. Overlay of currents recorded in same cell before and after LY294002 (10 μM) during a voltage step from −50 mV (HP) to 0 mV. Baseline current at −50 mV prior to voltage step to 0 mV was 0 nA. B. Effect of triciribine (10 μM) on Ca²⁺ current compared with control (mean ± SEM; n = 3). Holding potential = −50 mV.