Phosphoinositide-3-kinase/akt - dependent signaling is required for maintenance of [Ca2+]i,ICa, and Ca2+ transients in HL-1 cardiomyocytes

Table 3 Effect of phosphoinositide-3-kinases β and γ inhibitors on intracellular Ca ²⁺ concentration recorded 15 minutes after addition of respective inhibitors

Agent (Concentration) Target	Control [Ca²⁺]_i, (nM)	Inhibitor (15 min) [Ca²⁺]_i, (nM)	P value for difference
TGX-221 (100 nM) β	105.5 ± 5.0	96.6 ± 4.8	<0.03
AS-252424 (100 nM) γ	121.8 ± 4.3	111.9 ± 6.0	<0.03

Mean ± SEM (n = 5 experiments each, with each experiment comprising the average [Ca²⁺] of 25 to 40 cells).

ISSN: 1423-0127