Immunofluorescence staining for cTnI, connexin 43 and sarcomeric a-actin in induced BMMSCs. The induced cells were positive for cardiomyocyte-specific proteins cTnI (green fluorescence, panel A, Magnification: × 200), connexin 43 (green fluorescence, panel E, Magnification: ×400) and sarcomeric a-actin (red fluorescence, panel B, F, Magnification: B: ×200, F: × 400). The nuclei were counterstained with Hoechst 33258 (blue fluorescence, panel C, G, Magnification:C: ×200, G: × 400). The panels A, B and C were merged as D(Magnification: × 200). The panels E, F and G were were merged as H(Magnification: × 400).